Groundwater is a major source of water supply in urban centers like Kaduna Metropolis, where pipe borne water is not reliable in terms of availability and quality. This has necessitated the inhabitants of Kaduna Metropolis to seek for groundwater as an alternative. However human activities have resulted in the depletion of groundwater of this area. This study compares the impact of human activities on biological groundwater quality between North and South of Kaduna Metropolis. Fifty groundwater samples, comprising 30 from North and 20 from South (29 boreholes (motorized and hand pump) and 21 hand dug wells) were analysed for faecal and total coliform using membrane filter technique in accordance with America Public Health Association standard procedure. Result indicated that 60% and 70% of the groundwater in the South and North respectively is contaminated in faecal and total coliform.  The coliform count (cfu/100ml) in the water samples ranges between zero to “too numerous to count”. Coliform contamination is high in areas with poor sanitation and in locations where groundwater sources are installed close to toilet facilities as seen in the results. Hence, it is recommended that appropriate measures be taken to treat the groundwater by disinfection and boiling before drinking.

Keywords: Groundwater, Microbiological analysis, Human  activities, Pollution, Kaduna South and North